Instrumental liquid chromatography (HPLC method development/ UPLC method development) are commonly used in the pharmaceutical, environmental, and other areas for analyses that need to comply with either Good Laboratory Practices or ISO 17025 training.

In addition to the usual method validation steps that are required (such as the determinations of precision, accuracy, linearity, repeatability, selectivity), there are numerous environmental analysis steps specific to the validation of any HPLC methodology/ UPLC method. These include validation of the chromatograph; the detectors; the column – resolution, tailing, plate count; the injection system; the data system – the integration method and determination of a baseline; as well as the mobile phase components and other reagents utilized.

Why Should You Attend:

Quality assurances of these performances and ways to monitor them will be covered. An overview of the power of using control charts, and the statistically-based ways of using them to catch problems early on will also be reviewed.

Areas Covered in this Webinar:

Validation of:

  • The pumping system
  • The Injection system
  • The column
  • The detector(s)
  • The reagents and mobile-phase components
  • Data quality

Learning Objectives:

  • What things must be assessed and under control within a HPLC/UPLC methodology

Who Will Benefit:

  • Lab Chemists
  • Lab Managers
  • Lab Technicians
  • Lab Analysts
  • Industries into Compliance Methodology (Biotech, Pharma)
  • Companies into Environmental Compliance or EPA

Speaker Profile:

John C. Fetzer, has had over 30 year experience in HPLC methods development. He has authored or co-authored over 50 peer-reviewed papers onl iquid chromatography, has served on the editorial advisory boards of the Journal of Chromatography, Analytical Chemistry, and Analytical and Bioanalytical Chemistry.

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